In the special issue, we published a paper reporting that ozonation of cyanobacterial extract effectively removes microcystins, microcystin-independent in vitro tumor promoting activity (inhibition of gap junctional intercellular communication, activation of mitogen activated protein kinases) as well as cytotoxicity. In contract, chlorination was much less effective in removing toxins and in vitro toxicity, even if high chlorine doses causing a similar level of total organic carbon reduction as ozone were applied. The study indicates advantages of ozonation as well as effect-based monitoring in evaluation of water treatment technologies. Sovadinová, I.*, Babica, P., Adamovský O., Alpatova, A., Tarabara, V., Upham, B., Bláha, L. (2017): Effects of chlorination and ozonization on epigenetic toxicity of cyanobacterial extracts. Advances in Oceanography and Limnology 8(1) (in press) Full-text (Open Access)
Stoyneva-Gaertner, M. P.*, Pavlova, V., Uzunov, B., Bratanova, Z., Descy, J.-P., Babica, P., Marsalek, B., Meriluoto, J., Spoof, L. (2017): Assessment of cyanoprokaryote blooms and of cyanotoxins in Bulgaria in a 15-years period (2000-2015). Advances in Oceanography and Limnology 8(1) Full-text (Open Access) Pavel BBC
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Pavel BBC
A new study prepared by HEAR group at RECETOX (Anežka Sharma, Katarína Bányiová and Pavel Čupr) and their collaborators, including also Pavel Babica, has been published in Science of the Total Environment journal. The study has shown for the first time that photoisomerisation product cis-2-ethylhexyl 4-methoxycinnamate (cis-EHMC) formed by irradiation of a common sunscreen ingredient, trans-EHMC, is significantly more potent inducer of genotoxic damage in mammalian cells than the parental compound. These genotoxic effects were demonstrated not only in human lymphoblastoid TK-6 cells, but also in adult human liver stem cells HL1-hT1. Using QIVIVE modeling and probabilistic risk assessment, it has been demonstrated that cis-EHMC might represent up to 7-times higher health risk than trans-EHMC in selected populations, such as females.
A paper on the effects of nanosecond pulsed electric fields (nsPEFs) has been accepted for publication in Bioelectrochemistry journal. The paper was a result of collaboration between Prof. Juergen F. Kolb, Anna Steuer and Anke Schmidt at the Institute for Plasma Science and Technology, INP Greifswald (Germany), and RECETOX, Masaryk University (Czech Rep.), represented by Pavel Babica and Petra Labohá (a former postdoctoral researcher at Prof. Kolb's lab). The paper is the very first report on the modulations of gap junctional intercellular communication by nsPEFs, which is a very perspective tool being developed and tested for different biomedical applications, such as cancer therapy or wound healing. nsPEF treatments were inducing rapid, pronounced but reversible inhibition of GJIC in vitro accompanied by disassembly of connexin plaques and activation of mitogen-activated protein kinases. Modulations of GJIC by nsPEF might contribute to its biological effects, and could be utilized to further develop more efficient or specific nsPEF treatments. Steuer, A., Schmidt, A., Labohá, P., Babica, P., Kolb, J.F.* (2016): Transient suppression of gap junctional intercellular communication after exposure to 100-nanosecond pulsed electric fields. Bioelectrochemistry 112:33-46 UPDATE: Full-text PubMed Pavel BBC
There have been two new papers prepared with contribution of SECANTOX group members. Pavel Babica participated in a study focusing on oxidative transformation of estrogenic hormones by high-valent iron species. The study was done in collaboration among Institute of Botany (Department of Experimental Phycology and Ecotoxicology), Regional Centre of Advanced Technologies and Materials, and Texas A&M University (Department of Environmental and Occupational Health). Its results are going to be published in Physical Chemistry - Chemical Physics journal. The other paper co-authored by Pavel Babica and Libor Jaša focuses on cellular effects of well-recognized endocrine disrupting chemicals, methoxychlor and vinclozolin, which can apparently also alter intercellular and intracellular signaling via rapid mechanisms independent on genomic signaling by steroid hormone receptors. This study was prepared in collaboration between Institute of Botany, RECETOX and Michigan State University (Department of Pediatrics and Human Development and Institute for Integrative Toxicology) and accepted for publication in Toxicological Sciences. Machalová-Šišková, K., Jančula, D., Drahoš, B., Machala, L., Babica, P., Godoy-Alonso, P., Trávníček, Z., Maršálek, B., Sharma, V. K., Zbořil., R.* (2016): High-valent iron (FeVI, FeV, and FeIV) species in water: Characterization and oxidative transformation of estrogenic hormones. Physical Chemistry Chemical Physics 18(28):18802-18810 UPDATE: Full-text PubMed Babica, P.*, Zurabian, R., Kumar, E.R., Chopra, R., Mianecki, M. J., Park, J.-S., Jaša, L., Trosko, J. E., Upham, B. L. (2016): Methoxychlor and vincolozolin induce rapid changes in intracellular and intercellular signaling in liver progenitor cells. Toxicological Sciences 153(1):174-185 UPDATE: Full-text PubMed Pavel BBC
Two new papers co-authored by SECANTOX research group members have been accepted for publication. Both papers focus on the protocol for evaluation of gap junctional intercellular communication by scalpel (scrape) loading / dye transfer method. First paper will be the part of Springer's edition Methods in Molecular Biology (Vol. 1437) titled "Gap Junction Protocols" and edited by M. Vinken and S. Johnstone. The other paper will be published in Journal of Visualized Experiment. UPDATED: Upham, B. L.*, Sovadinová, I., Babica, P. (2016): Gap junctional intercellular communication: a functional biomarker to assess adverse effects of toxicants and toxins, and health benefits of natural products. Journal of Visualized Experiments 118:e54281 Full-text PubMed Babica, P.*, Sovadinová, I., Upham, B. (2016): Scrape loading / dye transfer. In: Methods in Molecular Biology Vol. 1437 - Gap Junction Protocols (Eds.: Vinken, M., Johnstone, S.), p. 133-144. Springer (ISBN 978-1-4939-3662-5). Full-text PubMed Pavel BBC
A new paper has been accepted for publication in Nutrition and Cancer journal. The paper focuses on the ability of various chemopreventive and anticancer agents to prevent in vitro inhibition of gap junctional intercellular communication (GJIC) induced by environmental toxicants and tumor promoters. It has been found that chemopreventive agents commonly block effects of GJIC inhibitors. Such activity may not only represent a crucial mechanism contributing to the beneficial effects of the well-recognized dietary or pharmaceutical chemopreventive agents, but it can be utilized as an endpoint in a high throughput in vitro screening for novel chemopreventive and anticancer chemicals. The study has been a result of the research project "Novel in vitro approach for identification of chemopreventive effects and mechanisms of phytochemicals" hosted by Institute of Botany (Department of Experimental Phycology and Ecotoxicology), and implemented in collaboration with Prof. Brad L. Upham and Prof. James E. Trosko (Michigan State University) and also SECANTOX research group of RECETOX (Pavel Babica, Lucie Čtveráčková and Zuzana Lenčešová): Babica, P.*, Čtveráčková, L., Lenčešová, Z., Trosko, J. E., Upham, B. L. (2016): Chemopreventive agents attenuate rapid inhibition of gap junctional intercellular communication induced by environmental toxicants. Nutrition and Cancer 68(5):827-837 Full-text (Open Access from this Link) PubMed Pavel BBC
Members of SECANTOX Research Group (Amrita Basu and Pavel Babica) contributed to a research study, which was just accepted for publication in Environmental Science and Technology journal. This study was a part of GACR research project "Immunomodulatory potencies of toxic cyanobacteria" supervised by Ondrej Adamovsky. The paper reports pro-inflammatory activity of cyanobacterial toxin microcystin-LR in a murine macrophage cell line, and for the first time indicates that microcystin effects in macrophages are probably mediated via activation of membrane toll-like receptors, independently of OATP-mediated cellular uptake of microcystins. Adamovský, O.*, Moosová, Z., Pekarová, M., Basu, A., Babica, P., Švihalková-Šindlerová, L., Kubala., L., Bláha, L. (2015): Immunomodulatory potency of microcystin, an important water polluting cyanobacterial toxin. Environmental Science and Technolology 49(20):12457–12464 Full-text PubMed Pavel BBC
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